利用混池重测序鉴定萨福克绵羊超数排卵关键调控基因

doi:10.11843/j.issn.0366-6964.2019.09.008

摘要/Abstract

摘要： 旨在对萨福克绵羊超数排卵处理基础上，通过基因组混池重测序鉴定影响超数排卵效果的关键调控基因。本研究对93头年龄在2~3岁之间、健康状况良好的萨福克供体绵羊进行超数排卵处理后，选取超数排卵效果良好（获得的胚胎数大于等于15枚）的30头个体组建高产群体，同时选取超数排卵效果不良（获得的胚胎数小于等于9枚）的30头个体组建低产群体。对2个群体进行基因组混池重测序，并对2个群体中发现的SNPs进行选择消除分析和基因富集分析。结果表明，超数排卵获得头均总胚胎数为（12.55±7.97）枚，头均可用胚胎数为（7.76±7.43）枚。构建的高产群体和低产群体平均可用胚胎分别为（14.97±8.38）枚和（2.27±2.10）枚。在高产群体和低产群体中分别检出20 189 224和20 396 751个SNPs，对这些SNPs进行选择消除分析和基因富集分析表明，共有11个候选基因能够影响萨福克绵羊超数排卵过程，其中7个基因属于HOXA基因家族，2个基因尚未被验证功能，剩余2个基因分别为DPH6和AKAP6。总体来讲，基于对萨福克绵羊超数排卵高产群体和低产群体的基因组混池重测序，共筛选得到11个关键调控基因，进一步验证HOXA基因家族、DPH6和AKAP6基因在卵泡发育中的作用将有助于解释家畜超数排卵调控机制。

Abstract: The aim of this study was to identify the key regulatory genes affecting superovulation traits by genome pool resequencing, based on the superovulation treatment on Suffolk sheep. A total of 93 Suffolk donor sheep, aged 2-3 years old with good health conditions, were treated with superovulation. Thirty individuals with good superovulation result (the number of embryos obtained was greater than or equal to 15) were selected to construct a high-yield group. At the same time, another thirty individuals with poor superovulation result (the number of embryos obtained was less than or equal to 9) were selected to construct a low-yield group. After the genome pool resequencing, the SNPs identified in the two groups were analyzed by the selective sweep and gene enrichment. The results indicated that the average total embryos for each individual obtained in the superovulation were (12.55±7.97), and the average viable embryos for each individual were (7.76±7.43). The average viable embryos for the constructed high-yield and low-yield groups were (14.97±8.38) and (2.27±2.10), respectively. A total of 20 189 224 and 20 396 751 SNPs were detected from the two groups by resequencing. After the selective sweep and gene enrichment analysis, a total of 11 candidate genes identified could affect the superovulation of Suffolk sheep, in which, seven genes belong to the HOXA family, the function of two genes have not been verified, and the other two are DPH6 and AKAP6. In general, based on the genome pool resequencing of the high-yield and low-yield groups in superovulation of Suffolk sheep, a total of 11 key regulatory genes were identified. Further validation of the role of HOXA gene family, DPH6 and AKAP6 genes in follicular development may be important to explain the regulation mechanism of superovulation in livestock.

中图分类号:

S826.3

滑留帅, 辛晓玲, 王璟, 罗生金, 曾滔, 巴提玛, 施巧婷, 徐照学, 王二耀. 利用混池重测序鉴定萨福克绵羊超数排卵关键调控基因[J]. 畜牧兽医学报, 2019, 50(9): 1813-1821.

HUA Liushuai, XIN Xiaoling, WANG Jing, LUO Shengjin, ZENG Tao, BA Tima, SHI Qiaoting, XU Zhaoxue, WANG Eryao. Identification of Key Regulatory Genes for Superovulation in Suffolk Sheep by Pool Resequencing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1813-1821.

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